Journal: Journal of Lipid and Atherosclerosis

Article Title: Factor XI Deficiency in apoE/FXI Double-Knockout Mice Decreases Atherosclerosis by Lowering MSR1 mRNA Expression Within the Plaque

doi: 10.12997/jla.2025.14.3.372

Figure Lengend Snippet: (A) Volcano plot illustrating gene expression in the atherosclerotic plaques of DKO mice (n=4) compared to apoE KO mice (n=5). Gray points represent genes with adjusted p -value <0.05; orange points represent genes with a fold change greater than 2 or less than 0.5 between the groups; and green points represent genes affected by both conditions. 64 genes were expressed differently in apoE/FXI DKO mice than in apoE KO mice. lists the biological pathways where these genes were found. (B) Heatmap of 45 significantly differentially expressed genes between the 2 genotypes (adjusted p -value <0.05, |log 2 FC| >1). DKO, double knockout; apoE, apolipoprotein E; KO, knockout; FXI, factor XI; log 2 FC, log 2 -fold change.

Article Snippet: ApoE KO mice on a C57BL/6 genetic background (Jackson Laboratory) and apoE/FXI DKO mice—created by crossbreeding apoE KO mice with FXI-deficient mice (FXI KO) on a C57BL/6 background (provided by Prof. David Gailani, Vanderbilt University, Nashville, TN, USA)— were maintained on a chow diet with water provided ad libitum.

Techniques: Gene Expression, Double Knockout, Knock-Out

Journal: Journal of Lipid and Atherosclerosis

Article Title: Factor XI Deficiency in apoE/FXI Double-Knockout Mice Decreases Atherosclerosis by Lowering MSR1 mRNA Expression Within the Plaque

doi: 10.12997/jla.2025.14.3.372

Figure Lengend Snippet: (A) Evaluation of leukocyte infiltration into the atherosclerotic plaque of apoE/FXI DKO and apoE KO mice (n=9) was performed using ImmQuant software. Each box represents the interquartile range, the bold line represents the median, the vertical lines represent the minimum and maximum, and the dots represent outlier values. Significant difference is indicated by an asterisk next to the cell type. (B) Sections representing the atherosclerotic plaque area in the aortic sinus after immunohistochemical staining with CD3 antibody (a marker of T cells). The images on the right are magnified (400×) views of the areas marked by squares in the pictures on the left (100×). apoE, apolipoprotein E; FXI, factor XI; DKO, double knockout; KO, knockout.

Article Snippet: ApoE KO mice on a C57BL/6 genetic background (Jackson Laboratory) and apoE/FXI DKO mice—created by crossbreeding apoE KO mice with FXI-deficient mice (FXI KO) on a C57BL/6 background (provided by Prof. David Gailani, Vanderbilt University, Nashville, TN, USA)— were maintained on a chow diet with water provided ad libitum.

Techniques: Software, Immunohistochemical staining, Staining, Marker, Double Knockout, Knock-Out

Journal: Journal of Lipid and Atherosclerosis

Article Title: Factor XI Deficiency in apoE/FXI Double-Knockout Mice Decreases Atherosclerosis by Lowering MSR1 mRNA Expression Within the Plaque

doi: 10.12997/jla.2025.14.3.372

Figure Lengend Snippet: MSR1 , macrophage scavenger receptor 1; DKO, double knockout; apoE, apolipoprotein E; KO, knockout; FXI, factor XI.

Article Snippet: ApoE KO mice on a C57BL/6 genetic background (Jackson Laboratory) and apoE/FXI DKO mice—created by crossbreeding apoE KO mice with FXI-deficient mice (FXI KO) on a C57BL/6 background (provided by Prof. David Gailani, Vanderbilt University, Nashville, TN, USA)— were maintained on a chow diet with water provided ad libitum.

Techniques: Double Knockout, Knock-Out